Product Description
| Description | Anti-KRas (G12S) Mouse Monoclonal Antibody |
| Another Name | Anti-KRas (Mutated G12S) Mouse Monoclonal Antibody |
| Catalog Number | NL25092 |
| Size: | 100 µl |
| Tested Applications: | ELISA WB IF ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:200 |
| Cited Applications: | WB IF |
| Tested Reactivity: | Human , mouse Rat |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: |
CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; ' C-K-RAS; c-Ki-ras; c-Ki-ras2; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1 |
| Immunogen : | A synthetic peptide from the internal region of KRas which includes the mutation of G12S, human origin |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 21 KDa |
| Observed mv: | 21 KDa |
| Uniprot: | P01116 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
|
Background: |
Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell surviva Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
|
Tested Applications: WB
Western blot analysis of recombinant RAS(G12S) and wildtype proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti RAS(G12S) monoclonal antibody (Cat. #NL25092).
Tested Applications: IF
Immunofluorescence of cells expressing RAS proteins with Anti RAS(G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti RAS(G12S) monoclonal antibody (Cat. #NL25092).
實驗方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
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| 免疫細胞化學實驗操作指南 Download protocol | |
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操作指南
| 免疫印跡實驗操作指南 Download protocol | |
| 免疫組化實驗操作指南 Download protocol | |
| 免疫沉淀實驗操作指南 Download protocol | |
| 免疫細胞化學實驗操作指南 Download protocol | |
| 免疫熒光實驗操作指南 Download protocol |
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